p smad2 (Bioss)
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p smad2
P Smad2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+smad2/Smad2(Ser465+%2B+Ser467)+Antibody/pmc13106170-115-52-56
Average 94 stars, based on 19 article reviews
P Smad2, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+smad2/Smad2(Ser465+%2B+Ser467)+Antibody/pmc13106170-115-52-56
Average 94 stars, based on 19 article reviews
p smad2 - by Bioz Stars,
2026-09
94/100 stars
Images
1) Product Images from "Molecular mechanism of Danshen injection in treating endometrial fibrosis induced by intrauterine adhesions via the LAMC2-CD44-TGF-β1-SMAD2/3 signaling pathway"
Article Title: Molecular mechanism of Danshen injection in treating endometrial fibrosis induced by intrauterine adhesions via the LAMC2-CD44-TGF-β1-SMAD2/3 signaling pathway
Journal: Frontiers in Physiology
doi: 10.3389/fphys.2026.1794215
Figure Legend Snippet: LPS induced activation of rat uterine fibroblasts. (A) WB detection of LAMC2 expression levels in epithelial cells, (B) Relative expression of LAMC2 in epithelial cells, (C) Level of LAMC2 in cells detected by ELISA in epithelial cells, (D) WB detection of E-cad, N-cad, α-SMA,TGF-β1, SMAD2/3 in fibroblasts, (E) Relative expression of E-cad, N-cad, α-SMA,TGF-β1, SMAD2, SMAD3 in fibroblasts, (F) Immunofluorescence staining of COL1A1 in fibroblasts, (G) Immunofluorescence staining of COL3A1 in fibroblasts, (H) Immunofluorescence staining of α-SMA in fibroblasts, (I) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA. Compared to model in fibroblasts, * P < 0.05, ** P < 0.01.
Techniques Used: Activation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Fluorescence
Figure Legend Snippet: Danshen injection improves endometrial fibrosis in IUA rats by inhibiting the LAMC2-CD44-TGF-β1- SMAD2/3 signaling pathway. (A) Uterine Weight, (B) Uterine Length, (C) Grading of intrauterine adhesions in each group, (D) Number of uterine glands, (E) Collagen volume fraction, (F) H&E Staining, (G) MASSON Staining, (H) Immunofluorescence staining of COL1A1, (I) Immunofluorescence staining of COL3A1, (J) Immunofluorescence staining of α-SMA, (K) Mean fluorescence intensity of COL1A1, COL3A1 and α-SMA, L: WB detection of LAMC2, TGF-β1, SMAD2, SMAD3, (L) Relative expression of protein LAMC2, TGF-β1, SMAD2, SMAD3. Compared to model, * P < 0.05, ** P < 0.01. For panels A-C, n = 6 independent animals per group; for panels (D–M) , n=3 independent animals per group.
Techniques Used: Injection, Staining, Immunofluorescence, Fluorescence, Expressing
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Article Title: Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4 Article Snippet: The protein concentration was adjusted using a BCA assay kit (Beyotime, Shanghai, China), following the manufacturer’s instructions. .. Protein samples were separated using 10% SDS-PAGE gel (Epizyme Biotech, Shanghai, China), and the proteins were transferred to membranes after electrophoresis at 150 V. The membranes were blocked with 5% skim milk powder and incubated overnight at 4 °C with the following primary antibodies: anti-FOXO3 (Proteintech, Wuhan, China, 1:2000 dilution), p-FOXO3 (Bioss, Beijing, China, 1:1000 dilution), anti-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), p-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), anti-Smad2 (Bioss, Beijing, China, 1:1000 dilution), Article Title: Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4 Article Snippet: The protein concentration was adjusted using a BCA assay kit (Beyotime, Shanghai, China), following the manufacturer’s instructions. .. Protein samples were separated using 10% SDS-PAGE gel (Epizyme Biotech, Shanghai, China), and the proteins were transferred to membranes after electrophoresis at 150 V. The membranes were blocked with 5% skim milk powder and incubated overnight at 4 ◦C with the following primary antibodies: anti-FOXO3 (Proteintech, Wuhan, China, 1:2000 dilution), p-FOXO3 (Bioss, Beijing, China, 1:1000 dilution), anti-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), p-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), anti-Smad2 (Bioss, Beijing, China, 1:1000 dilution), Article Title: The mutualistic relationship between M2c macrophages of TGFβ1 induction and gastric cancer cells: the correlation between protective mechanisms in the tumor microenvironment and polarization of subtypes of cells Article Snippet: .. After 2 h of closure with skimmed milk in TBST, the membranes were analyzed using TNF-α (Boster, A00002-5), IL-10 (Boster, A00021-2), TGFβ1 (Boster, BM4876), Smad2 (Immunoway, YP1185), SDS Page:Article Title: Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4 Article Snippet: The protein concentration was adjusted using a BCA assay kit (Beyotime, Shanghai, China), following the manufacturer’s instructions. .. Protein samples were separated using 10% SDS-PAGE gel (Epizyme Biotech, Shanghai, China), and the proteins were transferred to membranes after electrophoresis at 150 V. The membranes were blocked with 5% skim milk powder and incubated overnight at 4 °C with the following primary antibodies: anti-FOXO3 (Proteintech, Wuhan, China, 1:2000 dilution), p-FOXO3 (Bioss, Beijing, China, 1:1000 dilution), anti-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), p-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), anti-Smad2 (Bioss, Beijing, China, 1:1000 dilution), Article Title: Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4 Article Snippet: The protein concentration was adjusted using a BCA assay kit (Beyotime, Shanghai, China), following the manufacturer’s instructions. .. Protein samples were separated using 10% SDS-PAGE gel (Epizyme Biotech, Shanghai, China), and the proteins were transferred to membranes after electrophoresis at 150 V. The membranes were blocked with 5% skim milk powder and incubated overnight at 4 ◦C with the following primary antibodies: anti-FOXO3 (Proteintech, Wuhan, China, 1:2000 dilution), p-FOXO3 (Bioss, Beijing, China, 1:1000 dilution), anti-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), p-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), anti-Smad2 (Bioss, Beijing, China, 1:1000 dilution), Electrophoresis:Article Title: Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4 Article Snippet: The protein concentration was adjusted using a BCA assay kit (Beyotime, Shanghai, China), following the manufacturer’s instructions. .. Protein samples were separated using 10% SDS-PAGE gel (Epizyme Biotech, Shanghai, China), and the proteins were transferred to membranes after electrophoresis at 150 V. The membranes were blocked with 5% skim milk powder and incubated overnight at 4 °C with the following primary antibodies: anti-FOXO3 (Proteintech, Wuhan, China, 1:2000 dilution), p-FOXO3 (Bioss, Beijing, China, 1:1000 dilution), anti-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), p-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), anti-Smad2 (Bioss, Beijing, China, 1:1000 dilution), Article Title: Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4 Article Snippet: The protein concentration was adjusted using a BCA assay kit (Beyotime, Shanghai, China), following the manufacturer’s instructions. .. Protein samples were separated using 10% SDS-PAGE gel (Epizyme Biotech, Shanghai, China), and the proteins were transferred to membranes after electrophoresis at 150 V. The membranes were blocked with 5% skim milk powder and incubated overnight at 4 ◦C with the following primary antibodies: anti-FOXO3 (Proteintech, Wuhan, China, 1:2000 dilution), p-FOXO3 (Bioss, Beijing, China, 1:1000 dilution), anti-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), p-FOXO4 (Bioss, Beijing, China, 1:1000 dilution), anti-Smad2 (Bioss, Beijing, China, 1:1000 dilution), |